Technology Focus

Confocal microscopes use a pinhole to reject out of focus light resulting in optical sectioning and an improved signal to noise ratio when compared to widefield fluorescence imaging. (See figure below – only in-focus light can pass through the pinhole to reach the detector).

Confocal microscopes are ideal for imaging multi-labelled fluorescent samples up to around 100 microns in depth.  

Figure credit – https://www.edinst.com/

For increased scanning speeds, the Nikon AX R confocal has a Resonant scanner achieving speeds around 20x faster than standard Galvano scanning confocals. 

The field of view (FOV) on the Nikon AX R is larger as compared to our other available confocal systems. 

The water pump allows multi-area, timelapse and high-content plate screening with the high NA water objectives by maintaining the meniscus across the entire plate and/or preventing evaporation issues.