Resources
Featured
Sample Preparation and Clearing
Find out how refractive index matching and clearing can improve your image acquisition.
Nyquist calculator
Check your sampling against Nyquist – in practice, on biological samples, use 1.7x these values.
RMS Technical Tea Breaks
15 minute talks introducing important concepts in microscopy while you have a brew!
Microscopy Learning Platforms
- MicroscopyU (Nikon) – https://www.microscopyu.com/
- Zeiss Campus (Zeiss) – http://zeiss
- Science Lab (Leica) – https://www.leica-microsystems.com/science-lab
- Technical Resource Center (Olympus) – https://www.olympus-lifescience.com
- Technical Resources (Thorlabs) – https://www.thorlabs.com
Tutorials
- Microcourses – Microcourses are short educational videos on a variety of light microscopy topics, brought to you by a team of microscopists from Harvard Medical School.
- RMS Technical Tea Breaks – a series of short talks designed to help those who are new to microscopy to better design and implement your imaging experiments.
- iBiology Microscopy Series
- MyScope Microscopy Training https://myscope.training/index.html
Featured FAQ
Section coming soon
Recommended Reading
- Tutorial: guidance for quantitative confocal microscopy. Jonkman, J., Brown, C. M., Wright, G. D., Anderson, K. I., & North, A. J. (2020). Tutorial: guidance for quantitative confocal microscopy. Nature protocols, 15(5), 1585–1611. https://doi.org/10.1038/s41596-020-0313-9
- Clearing of fixed tissue: a review from a microscopist’s perspective. Silvestri, L., Costantini, I., Sacconi, L., & Pavone, F. S. (2016). Journal of biomedical optics, 21(8), 081205. https://doi.org/10.1117/1.JBO.21.8.081205
- Clarifying Tissue Clearing. Richardson, D. S., & Lichtman, J. W. (2015). Cell, 162(2), 246–257. https://doi.org/10.1016/j.cell.2015.06.067
- A guided tour into subcellular colocalization analysis in light microscopy. Bolte, S., & Cordelières, F. P. (2006). Journal of microscopy, 224(Pt 3), 213–232. https://doi.org/10.1111/j.1365-2818.2006.01706.x
- Image Processing for Biologists. Methods 2017. Editors – Richard Butler and Alex Sossick.
- Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition. North A. J. (2006). The Journal of cell biology, 172(1), 9–18. https://doi.org/10.1083/jcb.200507103
Microscopy Organisations
Royal Microscopical Society
Global BioImaging
EuroBioimaging
Training requests and equipment bookings are done through PPMS