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FPbase Spectra Viewer

Check the spectra of your fluorescent dyes and proteins.

Sample Preparation and Clearing

Find out how refractive index matching and clearing can improve your image acquisition.

Nyquist calculator

Check your sampling against Nyquist – in practice, on biological samples, use 1.7x these values.

RMS Technical Tea Breaks

15 minute talks introducing important concepts in microscopy while you have a brew!

Microscopy Learning Platforms


Featured FAQ

Section coming soon

Recommended Reading

  • Tutorial: guidance for quantitative confocal microscopy. Jonkman, J., Brown, C. M., Wright, G. D., Anderson, K. I., & North, A. J. (2020). Tutorial: guidance for quantitative confocal microscopy. Nature protocols15(5), 1585–1611.
  • Clearing of fixed tissue: a review from a microscopist’s perspective. Silvestri, L., Costantini, I., Sacconi, L., & Pavone, F. S. (2016). Journal of biomedical optics21(8), 081205.
  • Clarifying Tissue Clearing. Richardson, D. S., & Lichtman, J. W. (2015). Cell162(2), 246–257.
  • A guided tour into subcellular colocalization analysis in light microscopy. Bolte, S., & Cordelières, F. P. (2006). Journal of microscopy224(Pt 3), 213–232.
  • Image Processing for Biologists. Methods 2017. Editors – Richard Butler and Alex Sossick.
  • Seeing is believing? A beginners’ guide to practical pitfalls in image acquisition. North A. J. (2006). The Journal of cell biology172(1), 9–18.

Microscopy Organisations

Royal Microscopical Society

Global BioImaging

Training requests and equipment bookings are done through PPMS