Technology Focus

Light-sheet microscopy is a fluorescence technique that projects a thin ‘sheet’ of excitation light perpendicular to the direction of observation. Only the focus plane is illuminated making it gentler on the sample and thus ideal for long-term live imaging of transparent samples such as zebrafish or organoids. 

Image credit – Zeiss Lightsheet Z.1_workshop_UK distributable

Light-sheet microscopy is also well suited to imaging larger cleared samples because of the imaging speed. 

For tutorials on sample preparation and image aquisition on the Z1 Lightsheet microscope please visit the Zeiss Lightsheet Knowledge Base.

Cleared lung tissue sample prepared, imaged and stitched by Tessa Hughes. E-cadherin marks the epithelium and is shown in white.